LIN Chun-rong, ZHANG Cui-xiang, DONG Sha-sha, et al. Optimization of Extraction Technology and Investigation of Anti-oxidant Effects of Polysaccharides from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 46-49.
DOI:
LIN Chun-rong, ZHANG Cui-xiang, DONG Sha-sha, et al. Optimization of Extraction Technology and Investigation of Anti-oxidant Effects of Polysaccharides from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 46-49. DOI: 10.11653/syfj2013100046.
Optimization of Extraction Technology and Investigation of Anti-oxidant Effects of Polysaccharides from Stems of
Objective: To optimize extracting conditions of polysaccharides from stems of Megacarpaea delavayi
and investigate its anti-oxidation effects. Method: Polysaccharide in stems of M. delavayi was isolated by water extracting-alcohol precipitating method
taking extraction rate of polysaccharides as index
optimal extracting conditions were studied by orthogonal test.The content of polysaccharides was determined.by phenol-sulfuric acid method
moreover
in vitro activities on scavenging free radicals of ·OH and O2-· of the polysaccharide were also observed. Result: Optimal extraction conditions of polysaccharides were as followings:extracted 12 h with 15 times the amount of water at 80 ℃;under these conditions
the average yield of polysaccharides was 1.973% (RSD 1.87%)
the conversion factor between glucose and the polysaccharides was 1.127(n=5)
the content of polysaccharides was 80.17% (RSD 2.13%)in the crude polysaccharides.In addition
the polysaccharides had a certain effect on scavenging free radicals of ·OH and O2-· in vitro and exhibited the dose-effect relation. Conclusion: This optimized technology was stable and feasible with high extraction rate;Polysaccharides in stems of M. delavayi possessed a certain anti-oxidant effect.
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