Content Determination of Three Kinds of Benzene Glycosides in Different Parts of  from Different Areas by HPLC

CAI Hao; SUN Xiu-man; OUYANG Cai-jun; ZHANG Lei; XIE Zhi-yong; LIAO Qiong-feng

doi:10.11653/syfj2013100063

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Content Determination of Three Kinds of Benzene Glycosides in Different Parts of from Different Areas by HPLC

更新时间：2024-01-04

- Content Determination of Three Kinds of Benzene Glycosides in Different Parts of from Different Areas by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 10, Pages: 63-65(2013)
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- DOI：10.11653/syfj2013100063
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- Published：2013
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CAI Hao, SUN Xiu-man, OUYANG Cai-jun, et al. Content Determination of Three Kinds of Benzene Glycosides in Different Parts of from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 63-65.
DOI：

CAI Hao, SUN Xiu-man, OUYANG Cai-jun, et al. Content Determination of Three Kinds of Benzene Glycosides in Different Parts of from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 63-65. DOI： 10.11653/syfj2013100063.

摘要

目的: 采用RP-HPLC-DAD测定不同产地广东紫珠的叶与茎枝中金石蚕苷、毛蕊花糖苷及连翘酯苷B的含量。方法: 采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm

5 μm)

柱温30 ℃

流动相乙腈-0.1%乙酸水(20:80)

流速1.0 mL·min-1

检测波长330 nm。结果: 金石蚕苷、毛蕊花糖苷及连翘酯苷B依次在9.96～498

20.8～1004

10.1～505 mg·L-1与峰面积呈良好线性关系;三者平均加样回收率分别为101.3%(RSD 0.59%)

98.7%(RSD 1.1%)

104.2%(RSD 0.71%)。不同产地广东紫珠的叶与茎枝中金石蚕苷、毛蕊花糖苷及连翘酯苷B的质量分数范围分别为0.076%～1.87%

0%～3.19%

0.102%～1.59%。结论: 建立的方法准确、快速、重复性好

可用于同时测定广东紫珠中金石蚕苷、毛蕊花糖苷及连翘酯苷B的含量

为广东紫珠的质量控制提供实验依据。

Abstract

Objective: To develop a RP-HPLC-DAD method for determination of forsythiaside B

poliumoside

verbascoside in the leaves and stems of Callicarpa kwangtungensis from different habitats. Method: The separation was performed on a Phenomenex Luna C18 column(4.6 mm×250 mm

5 μm) with a mobile phase of acetonitrile-0.1% acetic acid(20:80)

the flow rate was 1.0 mL·min-1

the column temperature was 30 ℃ and the detecting wavelength was at 330 nm. Result: The linear ranges of forsythiaside B

poliumoside and verbascoside were 10.1-505

9.96-498

20.8-1004 mg·L-1

respectively;The average recoveries of three kinds of benzene glycosides were 104.2%(RSD 0.71%)

101.3% (RSD 0.59%)

98.69% (RSD 1.1%)

respectively.Samples of C. kwangtungensis from different habitats were analyzed

the results showed that content range of forsythiaside B

poliumoside and verbascoside were 0.102%-1.59%

0.076%-1.87%

0%-3.19%

respectively. Conclusion: This established method was rapid

accurate and repeatable.It could be used to simultaneously determinate the content of forsythiaside B

poliumoside and verbascoside in C. kwangtungensis and provide experimental basis for quality control of C. kwangtungensis.

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