Objective: To establish a method for determining the content of morphine in childhood cough mixture and cough mixture. Method: Samples were purified by solid phase extraction column and were determined by HPLC.Chromatography conditions were as followings:Agilent ZORBAX Eclipse XDB-C8 column(4.6 mm×250 mm

5 μm)

the mobile phase of 0.05 mol·L-1 KH2PO4-0.002 5 mol·L-1 sodium heptane-acetonitrile (18:18:5)

the detection wavelength 220 nm. Result: Morphine had a good linear relationship in the rang of 1.717-12.02 mg·L-1(r=0.999 8).The average recovery of childhood cough mixtrue was 99.4% (RSD 2.01%)

the average recovery of cough mixtrue was 100.9% (RSD 0.36%). Conclusion: This established method was simple and accurate with good reproducibility

it could be used to effectively control the content of morphine in childhood cough mixture and cough mixture.