ZHANG Hai-wei, ZHANG Yi, YANG Ji-jia, et al. Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 95-98.
DOI:
ZHANG Hai-wei, ZHANG Yi, YANG Ji-jia, et al. Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 95-98. DOI: 10.11653/syfj2013100095.
Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC
Objective: To determine the gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang by RP-HPLC and compare the content of different batches. Method: The separation was performed on a welchrom C18 (4.60 mm×250 mm
5 μm) column with gradient elution.The mobile phase was acetonitrile-0.25% methanoic acid solution
and the flow rate was 0.85 mL·min-1.The detection wavelength was set at 260 nm. The column temperature was kept at 25 ℃. Result: The linear ranges of gallic acid and ellagic acid were within 0.050 0-0.700 0
0.050 2-0.714 0 mg respectively. The average recoveries for gallic and ellagic acid were 103.22%
97.49% (n=6). Conclusion: This method is suitable for the determination of gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang. The results provide a basis for medicinal value research and product development of the traditional Tibetan drug Sanguotang.