Objective: To establish an HPLC method for content determination of gallic acid

salidroside

tyrosol and p-coumaric acid in Rhodiola Extract. Method: Phenomenex Luna C18 column (4.6 mm×250 mm

5 μm) was used with the mobile phase of acetonitrile-0.3% HAC at a flow rate of 0.7 mL·min-1. The detection wavelength was set at 275 and 308 nm and the column temperature was maintained at 40 ℃. Result: The linear ranges of gallic acid

salidroside

tyrosol and p-coumaric acid were in the range of 0.435-13.912 mg·L-1 (r=1

n=9)

22.813-730.00 mg·L-1 (r=0.999 8

n=6)

7.819-250.20 mg·L-1(r=0.999 8

n=6)

1.618-51.76 mg·L-1 (r=0.999 8

n=6). The average recoveries of four components were in accordance with the determination requirement. Conclusion: This method was simple

accurate and reproducible for the four components determination of the Rhodiola Extract.