Objective: To determine shikimic acid in Pinus khasys from different position. Method: The content of shikimic acid was determined by HPLC; the Agilent zorbax-C18 (4.6 mm×150 mm

5 μm) was used as analytical column

the mobile phase was consisted of methanol-1% phosphoric acid (3:97)

the flow rate was 0.8 mL·min-1

the detection wavelength was 213 nm

and the column temperature was 25 ℃. Result: Shikimic acid has a good line in the range of 0.05-0.8 μg (r=0.999 9); the average recovery was 99.00%

and with RSD 0.81% (n=9). Conclusion: The content of shikimic acid was different in P. khasys from different position

while the highest content of shikimic acid was 29.17 mg·g-1 in the pine needles

the pine needles can considered to be shikimic acid new resources exploited and utilized.