YAO Jiang-xiong, LI Chun, LIAO Duan-fang, et al. Study on HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 119-121.
DOI:
YAO Jiang-xiong, LI Chun, LIAO Duan-fang, et al. Study on HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 119-121. DOI: 10.11653/syfj2013100119.
Objective: To establish HPLC fingerprint of Morus alba
which was expected to be standards of quality control and identification of the Chinese crude drug. Method: The HPLC method was used
chromatography conditions were Agilent Eclipse XDB-C18(4.6 mm×250 mm
5 μm)column with gradient mobile phase of acetonitrile-0.1% acetic acid
UV detection wavelength was 290 nm and the column temperature was 30 ℃ with the flow rate of 1.0 mL·min-1
the sample injection was 10 μL. Result: HPLC fingerprints of 13 samples of M. alba were established.16 mutual peaks were selected as the fingerprint peaks in 11 samples.Among the obtained fingerprints
most of the detected peaks were separated effectively.11 samples had high similarities. Conclusion: The established HPLC fingerprint has desirable accuracy
repeatability and stability
which can be used for one of the quality control of M. alba.
Shandong Technical Innovation Center for Traditional Chinese Medicine Treatment of Respiratory Diseases,Shandong Hongjitang Pharmaceutical Group Co. Ltd.
Shandong Institute for Food and Drug Control
School of Pharmacy,State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine