Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC

CHEN Hong-ying; NI Jian; ZHANG Hui

doi:10.11653/syfj2013100122

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Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 10, Pages: 122-124(2013)
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- DOI：10.11653/syfj2013100122
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- Published：2013
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CHEN Hong-ying, NI Jian, ZHANG Hui. Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 122-124.
DOI：

CHEN Hong-ying, NI Jian, ZHANG Hui. Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 122-124. DOI： 10.11653/syfj2013100122.

摘要

目的: 建立复方野菊花含片中黄芩苷和蒙花苷含量测定的方法。方法: 采用HPLC

Kromasil-C18(4.6 mm×250 mm

5 μm) 色谱柱

流动相乙腈-0.1%磷酸(25:75)

检测波长334 nm。结果: 黄芩苷和蒙花苷进样量分别在0.460～2.760 μg (r=0.999 5)

0.020～0.400 μg (r=0.999 9)线性关系良好;黄芩苷和蒙花苷平均回收率分别为101.77% (RSD 0.98%)

98.36% (RSD 1.68%)。结论: 该方法简便易行

重复性好

可用于复方野菊花含片中黄芩苷和蒙花苷含量测定。

Abstract

Objective: To establish a RP-HPLC method for simultaneous determination of baicalin and buddleoside in Compound Yejuhua Buccal Tablets. Method: HPLC was adopted to determine the content of baicalin and buddleoside. The Kromasil-C18 Column (4.6 mm×250 mm

5 μm) was used with the mobile phase consisting of acetonitrile-0.1% aqueous phosphoric acid (25:75); the detection wavelength was set at 334 nm. Result: There was good linearity between the peak area and concentration within the range of 0.460-2.760 μg (r=0.999 9) for baicalin and 0.020-0.400 μg (r=0.999 9) for buddleoside respectively. The average recoveries of baicalin and buddleoside were 101.77% (RSD 0.98%) and 98.36% (RSD 1.68%) respectively (n=6). Conclusion: The method is simple

accurate and specific for quality control of Compound Yejuhua Buccal Tablets.

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Related Author

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GAO De-min

TIAN Jing-zhen

LI Xiao-ming

LUO Yu-jian

YUAN Yuan

HUANG Lu-qi

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Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

School of Traditional Chinese Medicine,Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

College Pharmacy of Xinjiang Medical University

The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine

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