Objective: To investigate the possible mechanism of Qingnao Yizhi(QNYZ)decoction on vascular dementia(VD) through testing the effect of vcerebrospinal fluid which contained Qingnao Yizhi decoction on synaptic remodeling so as to provide more scientific evidence for the clinical medication on VD patients. Method: The primary cultured rat cortical neurons were vaccinated in 96-well cell culture plates and the vaccination concentration was 1×106/mL.And the cells were divided into control group

H/R group

cerebrospinal fluid(CSF) group

high dose CSF containing QNYZ group(QNYZ-H)

low dose CSF containing QNYZ group(QNYZ-L) and CSF containing butylphthalide(NBP) group(except the control group

the other groups were firstly dealt with hypoxia for 24 hours and then received reoxygenation for 24 hours). The concentration of synaptic remodeling markers such as synapsin

microtubule associated protein 2 and growth associated protein-43 of the supernatant were assayed by the ELISA way. Result: The concentration of synaptic remodeling markers such as synapsin(SYN)

microtuble associated protein 2(MAP-2) and growth associated protein-43(GAP-43) of the supernatant of the QN-H

QN-L groups increased

and the three indices'level are obviously higher than these in the H/R group(P<0.01). Conclusion: Qingnao Yizhi decoction could improve the synaptic remodeling of the H/R CNC through regulating the expression of SYN

MAP-2 and GAP-43.