Objective: To study the influence of serum containing Guantong Fang and its compositions on apoptosis in rat vascular smooth muscle cells. Method: SD rats in each group were administered corresponding doses:Guantong Fang group(23.75 g·kg-1)

Huoxue Huayu(HXHY) group(8.75 g·kg-1)

Yiqi Yangyin(YQYY) group(5 g·kg-1)

Qingre Huatan(QRHT) group(10 g·kg-1) for 7 days. Then th serum containing drugs was prepared

VSMCs were divided into: blank control group (5% normal rat serum)

model group (5% normal rat serum and 1×10-6mol·L-1 AngⅡ)

Guantong Fang group(5% Guantong Fang Containing serum and 1×10-6mol·L-1AngⅡ)

HXHY group(5% HXHY Containing serum and 1×10-6mol·L-1AngⅡ)

YQYY group(5% YQYY Containing serum and 1×10-6mol·L-1AngⅡ)

QRHT group(5% QRHT Containing serum and 1×10-6mol·L-1AngⅡ)

After incubated 48h

cell apoptosis was evaluated by laser flow cytometry. Result: in the model group the early apoptosis rate of vascular smooth muscle cell was (0.36±0.14)%

late apoptosis (1.02±0.86)%

and in blank control group:early apoptosis (2.22±0.43)%

late apoptosis (1.29±0.57)%;In the Guantong Fang group:early (23.4±0.95)%

late (9.1±0.69)%

in HXHY group: early (8.08±0.54)%

late (18.6±1.48)%

in YQYY group early (16.9±0.79)%

late (4.38±0.90)%

in QRHT group early (5.9±0.99)%

late (11.5±0.7)%. Conclusion: Guantong Fang can inhibit the proliferation of VSMC and induce apoptosis; and effect of Guantong Fang group is better than its compositions.