Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression

FENG Jin-shan; WANG Shi-qun; YE Ying; ZHOU Xiao-ge; ZHENG Xue-bao

doi:10.11653/syfj2013100245

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Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression

更新时间：2024-01-04

- Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 10, Pages: 245-248(2013)
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- DOI：10.11653/syfj2013100245
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- Published：2013
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FENG Jin-shan, WANG Shi-qun, YE Ying, et al. Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 245-248.
DOI：

FENG Jin-shan, WANG Shi-qun, YE Ying, et al. Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 245-248. DOI： 10.11653/syfj2013100245.

摘要

目的: 检测与分析溃疡性结肠炎(UC)患者外周血CD19+CD5+B细胞(B1)及CD19+CD5+CD1d+B细胞(B10)的比例

探讨黄芩苷对其体外表达的影响。方法: 收集并分离活动期UC患者其外周血单核细胞(PBMC)

以年龄性别相匹配的正常人PBMC为对照

经脂多糖(LPS)+黄芩苷体外刺激淋巴细胞

流式细胞术检测刺激前后外周血B1和B10细胞比例。结果: UC患者外周血中B1和B10细胞占CD19+B细胞的比例(4.83%±3.26%

0.97%±0.48%)低于正常对照组(16.16%±11.89%

5.99%±3.59%)

P<0.05

LPS+黄芩苷体外刺激能上调UC患者B10淋巴细胞的比例(P=0.02)

但对正常人该淋巴细胞亚群未见明显影响。结论: B1和B10细胞可能在UC病程中发挥免疫调节作用

黄芩苷可能对UC患者的B10细胞有上调作用。

Abstract

Objective: The detection and analysis the proportion of CD19+CD5+B cells (B1) and CD19+CD5+CD1d+B cells (B10) in peripheral blood of patients with ulcerative colitis (UC)

exploring the effect of baicalin on up-regulating both the two subpopulations expression in vitro. Method: Separate peripheral blood mononuclear cells (PBMC) of UC patients

using the PBMCs of age and gender-matched helthy people as normal control

lymphocytes were stimulated by lipopolysaccharide(LPS)+baicalin in vitro

B1 and B10 cells proportion were detected by the flow cytometry before and after stimulation. Result: The proportion of B1 and B10 cells are decrease in UC patients peripheral blood compare to the normal control group(P=0.029

0.049). In vitro

LPS+baicalin stimulation raised the proportion of B10 lymphocytes of UC patients(P=0.02)

but no significant impact on normal subjects. Conclusion: UC may be related to the expression of B1 and B10 cells

baicalin might upregulates the proportion of B10 cells of UC patients.

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