Objective: To study the effect of raw Pinellia ternate and processed P. ternates on the secretion of inflammation cytokines of mice aorta endothelial cells. Method: Ligroin

acetidin

95% ethanol and water were used to extract the components in P. ternate

and these components were added to cells stimulated by lipopolysaccharide (LPS). Normal control groups(without drugs and LPS)

model groups(without drugs

with 10 mg·L-1LPS)and treatment groups (each with 10 mg·L-1LPS and different extracts of P. ternate

the dosages are 0.1

1

10

20 mg·L-1

respectively)were set up. After 24 h

the contentrations of interleukin-6 (IL-6) andtumor necrosis facor-alpha (TNF-α) in supernatant were tested by enzyme-linked immunosorbent assay (ELISA). Result: The extracts of P. ternate decreased the secretion of inflammation cytokines

significant differences were found in each treatment group(P<0.01 or P<0.05)

compared with control groups. The inhibitory effect of each composition of raw P. ternate on TNF-α was relevant to the dosage. At the highest dosage(20 mg·L-1)

the secretions of IL-6 and TNF-α were the lowest. Different processed P. ternates at the dosage of 20 mg·L-1 could decrease the secretion of TNF-α; the ligroin composition of the prepared P. ternate without adjuvant decreased IL-6 most. The water extracts of raw P. ternate and processed P. ternates could decrease the secretion of IL-6 and TNF-α; the ligroin composition of the prepared P. ternate without adjuvant decreased the secretion of inflammation cytokines most. Conclusion: The secretion of inflammation cytokines of mice aorta endothelial cells are inhibited by P. ternate to some extent.The effects are relevant to the processing methods and extract solvent.