Research on Protection Action of Five Ethanol-extractions of Traditional Chinese Medicine on Cardiac Microvascular Endothelial Cells Damaged by Oxidized Low Density Lipoprotein

WANG Lan; YANG Bin; LIANG Ri-xin; YIN Xiao-jie; YANG Wei-peng; WANG Yan-li; YANG Yan

doi:10.11653/syfj2013100276

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Research on Protection Action of Five Ethanol-extractions of Traditional Chinese Medicine on Cardiac Microvascular Endothelial Cells Damaged by Oxidized Low Density Lipoprotein

更新时间：2024-01-04

- Research on Protection Action of Five Ethanol-extractions of Traditional Chinese Medicine on Cardiac Microvascular Endothelial Cells Damaged by Oxidized Low Density Lipoprotein
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 10, Pages: 276-279(2013)
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- DOI：10.11653/syfj2013100276
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- Published：2013
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WANG Lan, YANG Bin, LIANG Ri-xin, et al. Research on Protection Action of Five Ethanol-extractions of Traditional Chinese Medicine on Cardiac Microvascular Endothelial Cells Damaged by Oxidized Low Density Lipoprotein[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 276-279.
DOI：

WANG Lan, YANG Bin, LIANG Ri-xin, et al. Research on Protection Action of Five Ethanol-extractions of Traditional Chinese Medicine on Cardiac Microvascular Endothelial Cells Damaged by Oxidized Low Density Lipoprotein[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 276-279. DOI： 10.11653/syfj2013100276.

摘要

目的: 观察茶叶、红花、黄芩、贯叶金丝桃、木蝴蝶等5种中药醇提取物对氧化低密度脂蛋白(ox-LDL)损伤的心肌微血管内皮细胞的保护作用

初步探讨其作用机制。方法: 建立体外培养大鼠心肌微血管内皮细胞ox-LDL氧化损伤模型

倒置显微镜下观察加入不同质量浓度的上述醇提物后

ox-LDL氧化损伤前后细胞形态学变化;以噻唑蓝(MTT)法检测各组细胞的增殖率;测定细胞上清液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果: 5种中药醇提物均能不同程度地提高内皮细胞存活率

提高培养液中SOD

GSH-Px活性

降低MDA含量。结论: 5种中药醇提物具有抗心肌微血管内皮细胞ox-LDL氧化损伤的作用

其机制可能与其提高机体抗氧化能力、抑制细胞脂质过氧化有关。

Abstract

Objective: To investigate the mechanism of protection action of 5 kinds of ethanol-extractions of traditional Chinese medicine (Camellia sinensis

Carthamus tinctorius; Scutellaria baicalensis; Hypericum perforatum; Oroxylum indicum) on cardiac microvascular endothelial cells damaged by oxidized low density lipoprotein (ox-LDL). Methed: The protection of 5 kinds of ethanol-extractions from traditional Chinese medicine and VitE were investigated after ox-LDL (100 mg·L-1) induced injury on the third generation of rat cardiac microvascular endothelial cells.Cell morphology before and after damaged by ox-LDL was observed under the inverted microscope after joining the different concentration of the above ethanol-extractions. Cellular viability was determined by MTT method. The supernatant was collected for detecting malondialdehyde (MDA)

superoxide dismutase (SOD)

glutathione peroxidase (GSH-Px) activity. Result: Five kinds of ethanol-extractions from traditional Chinese medicine increased the endothelial cells survival rate

reduced MDA contents and improved SOD

GSH-Px activity. Conclusion: The above ethanol-extractions of traditional Chinese medicine showed obvious anti-oxidative effect on cardiac microvascular endothelial cells damaged by ox-LDL. The anti-oxidative effect may be related to enhancing endogenous antioxidant activity and inhibiting cellular lipid peroxidation.

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