TAN Cheng-jia, MA Jia-hua, JIANG Qiao-mei, et al. Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 8-10.
DOI:
TAN Cheng-jia, MA Jia-hua, JIANG Qiao-mei, et al. Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 8-10. DOI: 10.11653/syfj2013110008.
Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin
Objective: To optimize purification technology of Catharsius molossus extract by DA201-C macroporous resin. Method: Single factor test was adopted to evaluate absorption capacity of DA201-C macroporous resin and some factors affecting purification technology
such as pH
eluting agent
flow rate and other factors.Automatic amino acid analyzer was used to analyze purified intermediates. Result: The best purification technology were as follows:the concentration of the sample solution 17.6 g·L-1
adsorption rate 2 BV·h-1
pH of the sample solution 4.0
eluted by 1 BV of deionized water and 4 BV of 70% ethanol
respectively.Desalination rate was up to 98.81%
the recovery rate of peptides in intermediates of C. molossus was up to 87.39%
the total content of bound amino acids (polypeptides) in alcohol eluate was 32.26%. Conclusion: This technology was simple
feasible and could be used for desalination and purification of intermediates of C. molossus.
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