Optimization of Trypsin Extraction Technology of Polysaccharides from by Orthogonal Test and BP Artificial Neural Network Model

SHAO Fang-yuan; FU Teng-fei; XUE Xing-yang; ZHOU Su-juan; CHEN Hong-yuan; MENG Jiang; WANG Ying-fang

doi:10.11653/syfj2013110014

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Optimization of Trypsin Extraction Technology of Polysaccharides from by Orthogonal Test and BP Artificial Neural Network Model

更新时间：2024-01-04

- Optimization of Trypsin Extraction Technology of Polysaccharides from by Orthogonal Test and BP Artificial Neural Network Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 14-17(2013)
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- DOI：10.11653/syfj2013110014
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- Published：2013
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SHAO Fang-yuan, FU Teng-fei, XUE Xing-yang, et al. Optimization of Trypsin Extraction Technology of Polysaccharides from by Orthogonal Test and BP Artificial Neural Network Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 14-17.
DOI：

SHAO Fang-yuan, FU Teng-fei, XUE Xing-yang, et al. Optimization of Trypsin Extraction Technology of Polysaccharides from by Orthogonal Test and BP Artificial Neural Network Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 14-17. DOI： 10.11653/syfj2013110014.

摘要

目的: 正交试验和BP神经网络模型优选胰蛋白酶法提取鱼腥草多糖的工艺条件。方法: 以多糖得率及其含量为指标

结合正交试验和人工神经网络方法

考察加酶量、温度、时间对提取工艺条件的影响。结果: BP神经网络模型优化工艺为加胰蛋白酶量2.5%

于45 ℃温浸2.4 h;正交试验优选的工艺条件为加胰蛋白酶量2%

于45 ℃温浸时间2.5 h;前者的综合评分略高于后者。结论: BP神经网络模型法优选的提取工艺耗时短、耗能低

在实际应用中具有较好成效。

Abstract

Objective: To optimize trypsin extraction technology of polysaccharides from Houttuynia cordata by BP-neural network model and orthogonal test. Method: With yield and content of polysaccharides as indexes

effect of the dosage of trypsin

time and temperature on extraction technology by orthogonal test and artificial neural network. Result: Optimum extraction technology of orthogonal test was as following:the dosage of trypsin 2%

soaked 2.5 h at 45 ℃;Optimum extraction technology of BP neural network model was as following:the dosage of trypsin 2.5%

soaked 2.4 h at 45 ℃

which was higher than orthogonal design in comprehensive score. Conclusion: Optimized extraction technology of BP neural network model had good results in practical applications for short time and low energy consuming.

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Related Institution

Natural History Museum of China

Beijing Key Laboratory of Chinese Materia Pharmacology，Institute of Basic Medical Sciences， Xiyuan Hospital，China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Chinese Materia Medica， Beijing University of Chinese Medicine

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