YAO Lin, GAO Fei, SUN Yan, et al. Optimization of Purification Technology of Kubai Fuyan Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 38-41.
DOI:
YAO Lin, GAO Fei, SUN Yan, et al. Optimization of Purification Technology of Kubai Fuyan Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 38-41. DOI: 10.11653/syfj2013110038.
Optimization of Purification Technology of Kubai Fuyan Suppositories
Objective: To optimize purification technology of Kubai Fuyan suppositories. Method: Macroporous resin method was adopted
with the content of berberine hydrochloride
matrine and osthole as index
type of macroporous resin and its purification technology was screened by single factor test.The content of indicators ingredients were determined by HPLC. Result: HPD-400A type macroporous resin was selected with optimum purification technology was as following:ratio of resin and crude drug 1: 3
the sample solution concentration of g·mL-1 with pH 8
elution flow rate of 1.5 BV·h-1
eluated with 5 BV water
then eluated with 5 BV of 20% ethanol and 40% ethanol
merged ethanol eluent
recovered
dried
then received purified extracts. Conclusion: Drug loading of suppositories increased 4.3 times after purified with HPD-400A type macroporous resin.This optimized purification technology was stable and feasible
which could be used for industrial production of Kubai Fuyan suppositories.
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Related Author
YUAN Yong
WANG Lu-mei
ZHANG Yong-jun
WANG Xin-chun
XIE Meng-ting
WANG Xiao-ke
WU Fu-hai
YUAN Yong1
Related Institution
Pharmacy College of Shihezi University
The First Affiliated Hospital of Medical College,Shihezi University
National Institutes for Food and Drug Control
Beijing Key Laboratory of Traditional Chinese Medicine(TCM) Quality Evaluation, Beijing University of Chinese Medicine
School of TCM, Beijing University of Chinese Medicine