Establishment of HPLC-ELSD Fingerprint of Ermu Granules

LU Jun-rong; FU Chao-mei; JI Ning-ping; HU Hui-ling; LIN Jun-zhi; XU Xiao-qiu

doi:10.11653/syfj2013110063

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Establishment of HPLC-ELSD Fingerprint of Ermu Granules

更新时间：2024-01-04

- Establishment of HPLC-ELSD Fingerprint of Ermu Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 63-66(2013)
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- DOI：10.11653/syfj2013110063
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- Published：2013
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LU Jun-rong, FU Chao-mei, JI Ning-ping, et al. Establishment of HPLC-ELSD Fingerprint of Ermu Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 63-66.
DOI：

LU Jun-rong, FU Chao-mei, JI Ning-ping, et al. Establishment of HPLC-ELSD Fingerprint of Ermu Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 63-66. DOI： 10.11653/syfj2013110063.

摘要

目的: 建立二母颗粒的HPLC-ELSD特征指纹图谱。方法: 采用HPLC-ELSD

选用Global Chromatography C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水梯度洗脱

流速1.0 mL·min-1

漂移管温度45 ℃

增益值1

雾化空气流速1.5 L·min-1。对10批二母颗粒样品进行HPLC-ELSD测定

并与知母药材、知母浸膏的HPLC-ELSD图谱进行对比

采用"中药色谱指纹图谱相似度评价系统"2004年版A进行相似度评价。结果: 10批二母颗粒样品与知母药材、知母稠膏的相似度较高(0.960~0.998)。结论: 二母颗粒的HPLC-ELSD指纹图谱能更加完善二母颗粒的质量评价体系

为其他制剂品种的"原药材-中间体-成品"的全过程控制研究提供参考。

Abstract

Objective: To establish HPLC-ELSD characteristic fingerprint for Ermu granules. Method: HPLC-ELSD was employed

separation was carried out on Global Chromatography C18column (4.6 mm×250 mm

5 μm)

mobile phase of water-acetonitrile with gradient elution

flow rate 1.0 mL·min-1

temperature of drift tube maintained at 45 ℃

the gain value 1

flow rate of atomizing air set at 1.5 L·min-1.Ten batches of Ermu granules were determined

and fingerprints of them were compared with raw materials of Anemarrhena asphodeloides and extracts of A. asphodeloides

similarity among them was analysed by 2004 Edition A of 'Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine'. Result: The similarity between ten batches of Ermu granules and raw materials of A. asphodeloides

extracts of A. asphodeloides were very high(0.960-0.998). Conclusion: HPLC-ELSD fingerprint of Ermu granules could make its quality evaluation system more completed

which could provide a reference for study of 'raw materials of Chinese medicine-intermediate materials-products' for the whole process control of other preparations varieties.

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Related Author

Yu-qing ZHANG

An WU

Ting ZOU

Zhi-feng ZHAO

Shao-hua XIAN

Yu XIANG

Xu-guang HU

Meng-nan ZHOU

Related Institution

School of Traditional Chinese Medicine， Guangdong Pharmaceutical University

Sinopharm Group Dezhong （Foshan） Pharmaceutical Co. Ltd.

Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Shenyang Pharmaceutical University

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