Study on Chemical Constituents and Antitumor Activity of

GU Qing; CAI Yin-na; YANG Guang-ming; CAI Bao-chang; PAN Yang

doi:10.11653/syfj2013110072

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Study on Chemical Constituents and Antitumor Activity of

更新时间：2024-01-04

- Study on Chemical Constituents and Antitumor Activity of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 72-75(2013)
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- DOI：10.11653/syfj2013110072
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- Published：2013
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GU Qing, CAI Yin-na, YANG Guang-ming, et al. Study on Chemical Constituents and Antitumor Activity of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 72-75.
DOI：

GU Qing, CAI Yin-na, YANG Guang-ming, et al. Study on Chemical Constituents and Antitumor Activity of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 72-75. DOI： 10.11653/syfj2013110072.

摘要

目的: 研究藏药镰形棘豆Oxytropis falcata的化学组成及其抗肿瘤活性。方法: 通过硅胶柱色谱、制备TLC分离纯化镰形棘豆的化学成分

通过NMR等波谱技术解析鉴定化合物结构

通过MTT法研究化合物1

5抑制人肝癌细胞HepG2、小鼠黑色素瘤细胞B16F10、人肝癌细胞SMMC-7721、人肝癌细胞HuH7以及人乳腺癌细胞MDA-MB-231的增殖影响。结果: 从镰形棘豆乙醇提取物中分离得到了5个化合物

分别为7-甲氧基二氢黄酮(7-methoxy flavanone

7-羟基二氢黄酮(7-hydroxy flavonone

4'-二羟基査耳酮(2'

4'-dihydroxy chalcone

4 '-二羟基-4'-甲氧基查尔酮(2'

4 '-dihydroxy-4'-methoxy chalcone

N-(2-苯乙基)肉桂酰胺(N-2-phenylethyl cinnamamide

5)。四甲基偶氮唑盐比色(MTT)结果表明化合物3具有较好的抗肿瘤活性

其IC50分别为68.60

169.67

105.88

175.18

172.37 μmol·L-1。结论: 其中化合物5为首次从该种属中分离得到

化合物3对5种肿瘤细胞的增殖具有明显的抑制作用。

Abstract

Objective: To study the constituents from Oxytropis falcate and their antitumor activity. Method: Constituent isolation and purification was carried by column chromatography and their structures were elucidated on the basis of spectral data analysis.Cytotoxicity of compounds 1

5 was assessed using MTT colorimetric method against HepG2

B16F10

SMMC-7721

HuH7 and MDA-MB-231 tumor cell lines. Result: Five compounds(1-5) were elucidated as 7-methoxy flavanone (1)

7-hydroxy flavonone (2)

4'-dihydroxy chalcone (3)

4'-dihydroxy-4'-methoxy chalcone (4) and N-2-phenylethylcinnamamide (5). The compounds 3 showed the strongest cytotoxic effect on these five cell lines with IC50 values of 68.60

169.67

105.88

175.18

172.37 μmol·L-1 respectively. Conclusion: Compound 5 was isolated from this genus for the first time. Compound 3 showed significant antitumor activity.

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