Objective: To establish an HPLC method for the simultaneous determination of chlorogenic acid

rutin

hyperoside

quercetin and kaempferol in Hypericum scabrum. Method: A high-performance liquid chromatography equipped a Sunfire C18 (4.6 mm×250 mm

5 μm) column with UV detector used. The mobile phase consisted of acetonitrile and 0.1% aqueous phosphoric acid with gradient elution. The detection wavelength was set at 360 nm and the flow rate was 1.0 mL·min-1. Result: The linear ranges of chlorogenic acid

rutin

hyperoside

quercetin and kaempferol were 0.01-0.61 μg (r=0.999 6)

0.10-2.02 μg (r=0.999 7)

0.10-1.01 μg (r=0.999 9)

0.10-1.24 μg (r=0.999 7)

0.005 7-0.17 μg (r=0.999 7)

the average recoveries were 98.87% (RSD 1.43%)

99.81% (RSD 0.86%)

99.98% (RSD 0.82%)

98.8% (RSD 1.73%)

99.85 (RSD 1.79%). Conclusion: The method is simple

accurate and repeatable

which can be used for quality control of H. scabrum.