Objective: To investigate the belonging of fingerprint peaks of HPLC fingerprints for Gardeniae Fructus and the prepared medical materials. Method: The chromatographic fingerprints were obtained by injecting the sample solution each time on a CenturySIL C18 BDS column (4.6 mm×200 mm

5 μm) with the gradient elution solvent system composed of 1% acetate acid -water and 1% acetate acid -acetonitrile. The flow rate was 1.0 mL·min-1

the column temperature was maintained at (30±0.15)℃ and the DAD detection wavelength was set at 265 nm. Result: 35 co-possessing peaks were selected as the fingerprint peaks by taking Geniposide peak as the reference peak. The fingerprint peaks were classified according to the characters of their UV-spectrum. The normalization value of fingerprint peak of Gardeniae Fructus and the prepared medical materials were calculated. Conclusion: The iridoid glycosides

organic acids and saffrons were the main compositions in Gardeniae Fructus

but their proportion varied among the crude drugs from different areas and their proportion also varied as they were prepared in different conditions.