Objective: To establish a method for simultaneously determining calycosin-7-O-β-D-glucoside and astragaloside Ⅳ in Astragali Radix by HPLC. Method: HPLC was performed on Waters symmetry C18 column (4.6 mm×250 mm

5 μm)

the mobile phase consisted of 30% acetonitrile and 70% water at a flow rate of 1 mL·min-1. The column temperature was set at 35 ℃

and the eluate was detected at 260 nm and 210 nm. Result: Quantitative analysis of HPLC showed that the linear ranges of calycosin-7-O-β-D-glucoside and astragaloside Ⅳ were 0.391 2-9.78

0.973 6-24.34 μg respectively. The average recoveries of two ingredients were 99.18% (RSD 2.43%) and 99.41% (RSD 2.59%). Conclusion: The method set up is simple and rapid

therefore available for the quality control of calycosin-7-O-β-D-glucoside and astragaloside Ⅳ in Astragali Radix.