Objective: To study the fingerprints of python oil by GC and establish the method of standard quality for python oil. Method: Methyl oleate was used as the internal standard substance. A Quartz capillary column was used (0.25 μm×30 m×0.25 mm). Injector temperature was set at 280 ℃. FID temperature was 280 ℃. The carrier gas flow rate is 0.8 mL·min-1. Distributary ratio was 20: 1.Incoming sample amount was 1 μL. The obtained data was analyzed by computer aided similarity evaluation and cluster analysis software. Result: There were 9 common peaks in 24 groups of python oil. The similarity degree between 24 python oil groups and standard python oil was found to be higher than 0.998.The range of abundance of reference peaks in 24 python oil groups was 0.454-0.493.The similarity and reference to the peak abundance distinguish the Python oil and other oil such as palm oil

peanut oil

lard oil and average snake oil. Conclusion: The method is simple

accurate

repeatable and reliable

which can be used as a quality control method for Python oil.