Determination of Puerarin in Chaiyin Oral Solution by HPLC

LI Xiang; LIU Gui-yang; MA Jian-li; ZHOU Liang

doi:10.11653/syfj2013110131

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Determination of Puerarin in Chaiyin Oral Solution by HPLC

更新时间：2024-01-04

- Determination of Puerarin in Chaiyin Oral Solution by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 131-133(2013)
- 作者机构：
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- DOI：10.11653/syfj2013110131
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- Published：2013
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LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Puerarin in Chaiyin Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 131-133.
DOI：

LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Puerarin in Chaiyin Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 131-133. DOI： 10.11653/syfj2013110131.

摘要

目的: 建立HPLC法测定柴银口服液中葛根素的含量。方法: Agilent Eclipse XDB-C18色谱柱(4.6 mm×150 mm

5 μm);流动相甲醇-水(25:75)

流速1.0 mL·min-1

检测波长250 nm

柱温30 ℃。结果: 在本试验条件下

葛根素与其他色谱峰分离良好

在0.020 64~0.825 6 μg与峰面积线性关系良好(r=0.999 9)

平均回收率100.44%

RSD1.45%(n=6)。结论: 方法简便快速、专属性强、灵敏度高、重复性好

可用于柴银口服液的质量控制方法。

Abstract

Objective: To establish an HPLC method for determination of puerarin in Chaiyin oral solution. Method: The Agilent Eclipse XDB-C18 (4.6 mm×150 mm

5 μm) was used

the mobile phase consisted of methanol-water (25: 75); the flow rate was 1.0 mL·min-1; the detection wavelength was set at 250 nm; the column temperature was kept at 30 ℃. Result: The puerarin peak was well separated with other peaks

and a good linear correlation was observed within the range of 0.020 64-0.825 6 μg (r=0.999 9). The average recovery was 100.44% with RSD 1.45% (n=6). Conclusion: The method is rapid and simple

it can be used for the quality control of Chaiyin oral solution with good selectivity

sensitivity and repeatability.

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Related Institution

Chengde Jingfukang Pharmaceutical Group Co.,Ltd.

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Jilin Agricultural University

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