Objective: To observe the effects of different concentrations of tetrahydroxy stilbene glycoside (TSG) on the growth and proliferation of neural stem cells(NSCs) which were injured by Aβ25-35

to discuss the protective effects of TSG on the NSCs injury induced by Aβ25-35. Method: NSCs were extracted on both sides of brain cortex from the embryos of 14 days of Kunming mice pregnancy

NSCs were cultured by Aβ25-35(25 μmol·L-1) to establish cell model of Alzheimer's disease (AD) and then were cultured with TSG. All cells were divided into 5 groups

control group; model group(Aβ25-35 25 μmol·L-1)

(TSG 1×10-8mol·L-1+Aβ25-35)group

(TSG 1×10-7 mol·L-1+Aβ25-35)group and (TSG 1×10-6mol·L-1+Aβ25-35)group.Using the chemical fluorescence staining method (EDU) to detect the proliferation of NSCs; applicating WST-1 cell proliferation and cell toxicity test to detect the activity of NSCs of different time and then after cultured 7 days in vitro

counting neurospheres was used to observe the update ability of NSCs. Result: TSG could protect NSCs from Aβ25-35 damage. Compared with model group

the morphology

reproduction rate

cell viability and ball count of NSCs were significant different from TSG group (P<0.05或P<0.01). Conclusion: TSG can protect the NSCs injury induced by Aβ25-35 in some ways.