Effect of Zuogui Wan on the Expression of c-Jun -terminal Kinase in Rats with Gentamicin-induced Renal Tubular Injury

FAN Wei-min; WANG Xiao-qin

doi:10.11653/syfj2013110194

您当前的位置：

首页 >

文章列表页 >

Effect of Zuogui Wan on the Expression of c-Jun -terminal Kinase in Rats with Gentamicin-induced Renal Tubular Injury

更新时间：2024-01-04

- Effect of Zuogui Wan on the Expression of c-Jun -terminal Kinase in Rats with Gentamicin-induced Renal Tubular Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 194-198(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013110194
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
FAN Wei-min, WANG Xiao-qin. Effect of Zuogui Wan on the Expression of c-Jun -terminal Kinase in Rats with Gentamicin-induced Renal Tubular Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 194-198.
DOI：

FAN Wei-min, WANG Xiao-qin. Effect of Zuogui Wan on the Expression of c-Jun -terminal Kinase in Rats with Gentamicin-induced Renal Tubular Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 194-198. DOI： 10.11653/syfj2013110194.

摘要

目的: 探讨左归丸对庆大霉素所致肾小管损伤大鼠c-Jun氨基末端激酶(JNK)的影响。方法: 将40只雄性Wistar大鼠随机分为空白对照组(C组)

模型组(M组)

c-Jun氨基末端激酶特异性抑制剂(SP600125)组(S组)

左归丸组(Z组)

每组10只

对照组ip给予0.9%生理盐水2.5 mL·kg-1·d-1

模型组ip硫酸庆大霉素100 mg·kg-1·d-1

SP600125组左侧ip硫酸庆大霉素100 mg·kg-1·d-1

2 h后右侧ip SP600125 15 mg·kg-1·d-1

左归丸组ip硫酸庆大霉素100 mg·kg-1·d-1和ig左归丸水溶液10 g·kg-1·d-1

4组大鼠连续给药10 d。第11日测量各组大鼠尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG);HE染色观察各组大鼠肾脏病理状况;免疫组化技术检测各组大鼠肾脏JNK

c-Jun的表达;以Western blot方法检测各组大鼠肾脏c-Jun氨基末端激酶(JNK)

c-Jun蛋白的表达。结果: 尿NAG酶结果显示:与C组比较

M组尿NAG酶显著升高(P<0.01);与M组比较

S组和Z组尿NAG酶下降(P<0.05)。HE染色结果显示:C组肾小管、间质无特殊改变;M组肾小管上皮细胞出现空泡变性、管腔出现损伤

有大量的炎性细胞浸润;S组与Z组肾小管少量炎性细胞浸润

轻微肿胀变性。免疫组化结果显示:与C组比较

M组JNK

c-Jun在肾小管大量表达(P<0.01);与M组比较

S组和Z组JNK

c-Jun在肾小管表达较少(P<0.01)。免疫印迹杂交结果显示:M组JNK

c-Jun蛋白灰度值较对照组明显升高(P<0.01)

S组与Z组较M组灰度值下降(P<0.01或P<0.05)。结论: 左归丸对庆大霉素所致肾小管损伤大鼠的保护作用可能与抑制c-Jun氨基末端激酶的激活有关。

Abstract

Objective: Effect of Zuogui Wan on the expression of c-Jun amino-terminal kinase(JNK) and c-Jun in rats with gentamicin-induced renal tubular injury. Method: Forty male Wistar rats were divided randomly into control group(C)

model group(M)

Sp600125 group(S) and Zuoguiwan group(Z). The C group was given ip injection of 0.9% saline 2.5 mL·kg-1·d-1

M group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1

S group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1 and ip injection of SP600125 15 mg·kg-1·d-1 Zuoguiwan group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1 and gavage Zuoguiwan aqueous solution 10 g·kg-1·d-1. Four groups of rats administered for 10 consecutive days. After the experiment measuring the NAG of rat. Hematoxylin-eosin to detect renal histopathology. Immunohistochemical techniques to detect JNK and c-Jun's expression of the rat kidney. Western blot techniques to detect JNK and c-Jun's expression of the rat kidney. Result: The results of biochemical index show: compared with the C group

the rats of M group NAG significantly increased

with a statistically significant difference (P<0.01); compared with the M group

the biochemical parameters improved of S group and Z group

with a statistically significant difference (P<0.05). The HE staining results show: the C group of renal tubule

renal interstitial no change; the M group of renal tubular epithelial cells swelling

vacuolar degeneration. The S group and Z group renal tubule epithelial cells slight swelling

degeneration. The immunohistochemical results show: compared with the C group

the rats of M group JNK

c-Jun abundantly expressed in the renal tubules

with a statistically significant difference (P<0.01); compared with the M group

protein expression was not significant in the S group and Z group

with a statistically significant difference (P<0.01). The results of western blot show: compared with the C group

the rats of M group JNK

c-Jun's gray values increased significantly

with a statistically significant difference (P<0.01); compared with the M group

the gray values of S group and Z group decreased

with a statistically significant difference (P<0.05

P<0.01). Conclusion: The protective effect of Zuogui Wan relieved the gentamicin-induced renal tubular injury in rats

which mechanism may be involved in the inhibition of the c-Jun N-terminal kinase activation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Zuogui Wan and Yougui Wan on Mitochondrial Biogenesis in BMSCs Through PGC-1α/PPARγ

Prevention and Treatment of Renal Tubular Injury in Diabetic Kidney Disease with Traditional Chinese Medicine： A Review

Type I and Ⅱ Cardiorenal Syndrome-related Biomarkers

Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC

Related Author

REN Yanling

GUO Xian

WANG Zhimin

CHEN Yiran

FENG Xiuzhi

YANG Ying

HUANG Guodong

ZHOU Yan

Related Institution

Liaoning University of Traditional Chinese Medicine

Affiliated Hospital of Liaoning University of Traditional Chinese Medicine

Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica， Guangxi University of Chinese Medicine

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine(TCM)

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰