Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA

GAO Run-cheng; WANG Zhi-wen; YUAN Qiang; ZHANG Ai-guo; YANG Yu-yang; LI Yi-liang; XIANG Nan

doi:10.11653/syfj2013110228

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Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA

更新时间：2024-01-04

- Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 228-231(2013)
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- DOI：10.11653/syfj2013110228
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- Published：2013
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GAO Run-cheng, WANG Zhi-wen, YUAN Qiang, et al. Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 228-231.
DOI：

GAO Run-cheng, WANG Zhi-wen, YUAN Qiang, et al. Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 228-231. DOI： 10.11653/syfj2013110228.

摘要

目的: 观察兔膝骨关节炎(KOA)软骨组织形态及p38丝裂原活化蛋白激酶(p38 MAPK)、半胱氨酸天冬氨酸特异性蛋白酶3(Caspase-3)的表达

探讨麝香乌龙丸治疗兔KOA的作用机制。方法: 将40只成年雄性大耳白兔

随机分为正常组(A)10只

造模组30只。采用关节腔内注射1.6%木瓜蛋白酶方法建立兔KOA模型

造模成功后随机分为模型组(B)、麝香乌龙丸低、高剂量组(C

n=10)

ig给予麝香乌龙丸0.6

1.2 g·kg-1·d-1

灌胃4周后处死动物切取软骨组织

观察软骨组织形态变化

免疫组化染色检测关节软骨细胞中p38MAPK

Caspase-3的表达。结果: 麝香乌龙丸低、高剂量组均能减轻兔软骨的病理损害

与模型组比较差异有统计学意义(P<0.05);麝香乌龙丸低、高剂量组均能降低软骨中p38MAPK及Caspase-3表达

与模型组比较具有显著差异(P<0.01)。结论: 麝香乌龙丸能够减轻兔骨关节炎软骨病理损害;麝香乌龙丸治疗骨关节炎的机制

与其能降低p38MAPK

Caspase-3的表达

抑制软骨细胞过度凋亡有关。

Abstract

Objective: To study expression of p38 mitogen-activated proteikinase(p38 MAPK) and Caspase-3 in cartilage of the rabbit of knee osteoarthritis (KOA)

and discuss the possible mechanisms by Shexiang Wulong pill in treatment of osteoarthritis. Method: Forty only adult male rabbits were randomly divided into 4 group he control group

the model group

high dose group and low dose group of Shexiang Wulong pill (n=10). 1.6% papain protein was injected to the rabbit knee joint cavity to establish rabbit knee osteoarthritis model

Shexiang Wulong pill groups were ig treated with Shexiang Wulong pill 0.6

1.2 g·kg-1·d-1 for 4 weeks. To observe the pathomorphology of cartilage HE staining was used and the expression levels of p38MAPK and caspase-3 was detected by immunohistochemistry technology. Result: Shexiang Wulong pill could alleviate the rabbit cartilage pathological changes. Compared with the model group

Shexiang Wulong pill could reduce the expression of p38MAPK and Caspase-3 in cartilage tissue (P<0.01). Conclusion: The Shexiang Wulong pill may reduce expression of p38MAPK and Caspase-3

and inhibit excessive cartilage cell apoptosis-related.

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