Objective: To study the antioxidant activity of oligopeptide isolated from Short-neck clam (Ruditapes philippinarum) muscle hydrolysate. Method: The antioxidant activities were evaluated by using 2

2'-azinobis -diammonium salt (ABTS)

hydroxyl

and superoxide anion radical scavenging assay

and Fe2+ chelation assay. The anti-prostatic cancer activity was evaluated by MTT assay. Result: Ruditapes philippinarum oligopeptide exhibited potent scavenging activity on ABTS radical (EC50 0.204 g·L-1)

superoxide anion (EC50 0.066 g·L-1)

and hydroxyl radical (EC50 0.815 g·L-1)

and significient Fe2+ chelation (EC50 1.554 g·L-1) ability and proliferation inhibition ability (IC50 5.202 g·L-1) of DU-145 cells. Conclusion: The antioxidant and anti-prostatic cancer activities of oligopeptide were due to the small molecular weight and special oligopeptide sequence.