On the Glycosides of  Caused Reproductive Damage Male Juvenil Rats Serum Testosterone and Testicular Tissue P450scc and the Prevention Effect of Bushen Chinese Herbs

JING Xiao-ping; HE Li

doi:10.11653/syfj2013110242

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On the Glycosides of Caused Reproductive Damage Male Juvenil Rats Serum Testosterone and Testicular Tissue P450scc and the Prevention Effect of Bushen Chinese Herbs

更新时间：2024-01-04

- On the Glycosides of Caused Reproductive Damage Male Juvenil Rats Serum Testosterone and Testicular Tissue P450scc and the Prevention Effect of Bushen Chinese Herbs
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 242-245(2013)
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- DOI：10.11653/syfj2013110242
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- Published：2013
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JING Xiao-ping, HE Li. On the Glycosides of Caused Reproductive Damage Male Juvenil Rats Serum Testosterone and Testicular Tissue P450scc and the Prevention Effect of Bushen Chinese Herbs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 242-245.
DOI：

JING Xiao-ping, HE Li. On the Glycosides of Caused Reproductive Damage Male Juvenil Rats Serum Testosterone and Testicular Tissue P450scc and the Prevention Effect of Bushen Chinese Herbs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 242-245. DOI： 10.11653/syfj2013110242.

摘要

目的: 研究雷公藤多苷(glycosides of Tripterygium wilfordii

GTW)对幼年雄性大鼠血清睾酮及睾丸组织中睾酮生成酶细胞色素P450胆固醇侧链裂解酶(cyto-chrome P450 side chain cleavage

P450scc)的影响以及补肾中药(菟丝子黄酮、六味地黄丸)对其损伤的干预作用。方法: 3~4周龄SD雄性幼鼠48只

随机分为4组:空白组(CMC-Na)、多苷组(9 mg·kg-1·d-1)单独ig

六味组(六味地黄丸1 g·kg-1·d-1＋GTW 9 mg·kg-1·d-1)

黄酮组(菟丝子黄酮0.1 g·kg-1·d-1＋GTW 9 mg·kg-1·d-1)混合ig

持续给药12周后

处死大鼠留取血清和睾丸组织

采用放免法检测血清睾酮浓度;采用RT-PCR和Western blot检测睾丸组织中p450scc在基因和蛋白水平的表达。结果: GTW组与空白组相比可以明显降低血清睾酮(P<0.01);六味组与多苷组相比可以明显提高血清睾酮(P<0.05);在蛋白水平GTW与空白组相比可以明显降低睾丸组织P450scc(P<0.05);在基因水平GTW组与其他组比较无显著性差异。结论: GTW对幼年大鼠生殖系统有一定损伤作用;六味地黄丸可以通过提高雄鼠血清睾酮水平而达到保护生殖损伤的作用。

Abstract

Objective: The research that the juvenile male juvenil rats serum testosterone and testicular tissue (cyto-chrome P450 side chain cleavage

P450scc) was impacted by Tripterygium wilfordii glycosides (GTW) and its intervention role Bushen Chinese herbs (flavonoids from Cuscuta chinensis

Liuwei Dihuang Wan). Method: SD 3-4 week-old 48 male rats were randomly divided into 4 groups: control group(CMC-Na)

GTW(9 mg·kg-1·d-1)

Liuwei Dihuang Wan Group (Liuwei Dihuang Wan 1 g·kg-1·d-1＋GTW 9 mg·kg-1·d-1)

flavonoids from C.chinensis group(flavonoids from C.chinensis 0.1 g·kg-1·d-1＋GTW 9 mg·kg-1·d-1).After 12 weeks

rats were sacrificed

the blood serum was collected from the rats for the determination of serum testosterone;specimens of testicular tissue for the detection of P450scc in the expression of the gene and protein level by two experimental methods using RT-PCR and Western blot. Result: GTW can significantly decrease serum testosterone values

GTW group and blank group comparison there is a significant difference (P<0.01)

Liuwei group with GTW group can significantly increase serum testosterone values

Liuwei group and GTW group comparison GTW group have significant significant difference (P<0.05);GTW at the protein level can significantly decrease the testicular tissue P450scc value

GTW group P450scc protein expression values were lower than other groups

GTW group and blank group comparison have significant difference (P<0.05); GTW cannot reduce the testicular tissue P450scc value at the genetic level

GTW group and other groups showed no significant difference. Conclusion: Prompted GTW certain inhibitory effect on the reproductive system of male juvenil rats; prompted Liuwei Dihuang Wan can significantly improve male rats serum testosterone levels and achieve the role of protecting reproductive damage.

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Related Institution

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