CAI Feng-jing, CHEN Xiang-rong, XU Zhao-yang, et al. Triptolide Inhibits Proliferation and Induces Apoptosis of C6 Glioma Cells by Regulating the Expressions of Bcl-2 and Bax[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 246-249.
DOI:
CAI Feng-jing, CHEN Xiang-rong, XU Zhao-yang, et al. Triptolide Inhibits Proliferation and Induces Apoptosis of C6 Glioma Cells by Regulating the Expressions of Bcl-2 and Bax[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 246-249. DOI: 10.11653/syfj2013110246.
Triptolide Inhibits Proliferation and Induces Apoptosis of C6 Glioma Cells by Regulating the Expressions of Bcl-2 and Bax
Objective: To investigate the effect of triptolide (TPL) on proliferation and apoptosis of C6 cells. Method: C6 cells was cultured with different concentrations of TPL for different hours. MTT assay was employed to evaluate the survival of TPL-treated C6 cells. The induction of apoptosis was detected by flow cytometry analysis (FCM). The expressions of B cell lymphoma/lewkmia-2(Bcl-2) and Bcl-2 assaciated x protein(Bax) mRNA were detected by Real Time - Polymerase Chain Reaction (RT-PCR)
while the protein by Western blotting (WB) methods. Result: Our results showed that TPL induced apoptotic cell death in a dose- and time-dependent manner
which was accompanied by the regulation of Bcl-2 and Bax expressions. Conclusion: The results indicated that TPL induce the apoptosis and inhibit the proliferation of C6 glioma cellsby regulating the expressions of Bcl-2 and Bax.
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