Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1, Th2 in Mice Infected by Influenza Virus

REN Liang; SONG Su-hua; JIANG Lu; LOU Zheng-chi; SONG Rong-qiang; LIU Yong; LV Cui-xia

doi:10.11653/syfj2013110255

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Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1, Th2 in Mice Infected by Influenza Virus

更新时间：2024-01-04

- Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1, Th2 in Mice Infected by Influenza Virus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 11, Pages: 255-260(2013)
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- 作者简介：
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- DOI：10.11653/syfj2013110255
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- Published：2013
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REN Liang, SONG Su-hua, JIANG Lu, et al. Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1, Th2 in Mice Infected by Influenza Virus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 255-260.
DOI：

REN Liang, SONG Su-hua, JIANG Lu, et al. Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1, Th2 in Mice Infected by Influenza Virus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 255-260. DOI： 10.11653/syfj2013110255.

摘要

目的: 探讨清营解表合剂对流感病毒感染小鼠免疫功能的影响。方法: 以流感病毒亚洲甲型鼠肺适应株(A/PR/8/H1N1)感染小鼠为模型

灌服清营解表合剂16 g·kg-1(0.8 g·mL-1

0.4 mL/只)

1次/d

连续ig给药12 d

应用流式细胞仪分析药后3

12 d特异性细胞因子γ-干扰素

肿瘤坏死因子-α

白细胞介素3

10的动态表达水平及Th1(IFN-γ)/Th2(IL-4)值。结果: 流感病毒感染小鼠后3 d

细胞因子IFN-γ升高显著(P<0.05)

12 d无显著改变

9 d较3 d均降低显著(P<0.05);3

12 d 细胞因子TNF-α均升高显著(P<0.01

P<0.05);3

12 d细胞因子IL-3均升高

6 d差异显著(P<0.05);3

12 d细胞因子IL-4

IL-6

IL-10 均显著升高(P<0.01)

在早期3 d达到峰值;3

12 d外周血Th1(IFN-γ)/Th2(IL-4)明显降低(P<0.01

P<0.05)

且6 d降到最低点

12 d较6 d升高显著(P<0.05)。经清营解表合剂治疗后

3 d细胞因子IFN-γ水平升高

较模型组无显著差异

12 d较模型组升高显著(P<0.01

P<0.05);3

12 d细胞因子 TNF-α水平表达较模型组均明显下降(P<0.01

P<0.05);3

12 d细胞因子IL-3 较模型组均有所下降

但差异无统计学意义;3

12 d细胞因子IL-4

IL-6

IL-10 水平较模型组均显著降低;3

12 d 外周血Th1(IFN-γ)/Th2(IL-4)均升高

差异显著(P<0.01)

12 d清营解表组较3 d升高显著(P<0.01)。结论: 清营解表合剂通过调节流感病毒感染小鼠外周血Th型细胞因子的表达水平

恢复机体的抗感染免疫平衡

提高机体免疫功能

以防治流感病毒感染。

Abstract

Objective: To investigate the influence of Qingying Jiebiao mixture (QJM) on immune function in mice infected by flu virus. Method: The model of influenza virus in mice was established by lung infection with Asia line(A/PR/8/H1N1). QJM suspension (0.8 g·mL-1) 0.4 mL was orally given to each mouse (16 g·kg-1) daily for 12 days. After administration of 3

9 d and 12 d

the expression level of specific cytokine interferon-gamma

tumor necrosis factor-α

interleukin 3

the ratio of Th1 (interferin-γ

IFN-γ) and Th2 (interleukin-4

IL-4) were determined by flowcytometry. Result: After the mice was infected with influenza virus

cytokines IFN-γ at 3 d was increased significantly (P<0.05)

those at 6

9 d and 12 d showed no significant changes. IFN-γ at 6

9 d was decreased significantly compared with that at 3 d (P<0.05); and cytokine tumar necrosis factoi-α(TNF-α) at 3

12 d were increased significantly (P<0.01

P<0.05); cytokines IL-3 at 3

12 d was increased

those at 3

6 d showed significant difference (P<0.05). Cytokines IL-4

IL-6 and IL-10 at 3

12 d were significantly increased (P<0.01)

and reached the peak in the early 3 d. Peripheral blood Th1 (IFN-γ)/Th2 (IL-4) at 3

12 d were decreased obviously (P<0.01

P<0.05)

and dropped to the lowest point at 6 d

and then increased significantly at 12 d compared with that at 6 d (P<0.05). After treatment of QJM

cytokines IFN-γ at 3 d was slightly increased

without significance to the model

however those at 6

12 d were increased significantly (P<0.01

P<0.05) compared to the model. Cytokines level of TNF-α expression at 3

12 d were significantly decreased compared to those in the model group (P<0.01

P<0.05). Cytokine IL-3 at 3

12 d was slightly decreased

without significance to the model. Cytokines IL-4

IL-6

IL-10 at 3

12 d were significantly lower than those in the model group. Peripheral blood Th1 (IFN-γ)/Th2 (IL-4) at 3

12 d were significantly increased (P<0.01)

and that at 12 d was increased significantly compared with that at 3 d (P<0.01). Conclusion: QJM can control the infection of influenza virus through regulating Th type cytokine expression in infected mice

improving immune function and restoring the resistance to infection.

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