LAI Li-na, REN Ze-en, SONG Xiao-liang, et al. Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 264-267.
DOI:
LAI Li-na, REN Ze-en, SONG Xiao-liang, et al. Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 264-267. DOI: 10.11653/syfj2013110264.
Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis
Objective: To investigate the effects of artesunate(Art) on Matrix Metalloproteinase-2(MMP-2) activity
explore the mechanism against liver fibrosis. Method: Liver fibrosis model of rats was induced by injecting N-nitroso dimethylamine (DMN) for 6 weeks. Rats were randomly divided into 6 groups: the normal group
the model group
the positive control group treated by colchicine(0.1 mg·kg-1·d-1)
and the three Art groups(5
15
45 mg·kg-1·d-1). The content of hydroxyproline (Hyp) of liver tissue
hyaluronic acid (HA)
laminin (LN)
collagen type Ⅲ peptide (PⅢP) of serum were analyzed. Expressions of MMP-2 protein and mRNA were assayed by Western blot and RT-PCR. Result: The content of Hyp in liver and HA
LN
PIIIP of serum in artesunate group were remarkably decreased and expressions of MMP-2 protein and mRNA were down-regulated compared with the model group. Conclusion: Artesunate can effectively inhibit the progress of hepatic fibrosis and the expression of MMP-2 in rats of liver fibrosis induced by DMN.