Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells
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Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 11, Pages: 272-275(2013)
DING Ning, YANG Yu-fei, LIU Yi-nan, et al. Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 272-275.
DOI:
DING Ning, YANG Yu-fei, LIU Yi-nan, et al. Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 272-275. DOI: 10.11653/syfj2013110272.
Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells
Objective: To investigate the impacts of the drug-containing serum of decomposed formulas based on Jianpi Huoxue Jiedu method on growth
rate of apoptosis
apoptosis related genes and autophagy gene expression t in human colorectal cancer cell line HCT-116 cells. Method: The experiment was divided into a control group
Jianpi group
Huoxue group
Jiedu group
Jianpi+Huoxue group Jianpi+Jiedu group and Jiapi+Huoxue+Jiedu group respectively
using the acid phosphatase enzymatic (APA)
flow cytometry (FCM) and Western blot to assay each drug-containing serum' treated tumor cell's growth
apoptosis rate changes
apoptosis-related genes Caspase-3 expression and autophagy LC3 gene expression. Result: After 10% drug-containing serum treated for 24 h
it showed inhibited proliferation of HCT-116 cells
however
no significant difference between the groups was found. The decrease in cell number and the shrinking in volume could be observed by light microscope after drug-containing serum treated for 24 h. Early and late apoptotic rate compared with the control group showed no significant difference
but autophagy related protein LC3 appeared active shear bands. Conclusion: The drug-containing serum of Jianpi Huoxue Jiedu method can inhibit human colorectal cancer cell line HCT-116 cells on proliferation in vitro. The mechanism may be closely related to the autophagy pathway.