BAI Dong, WANG Rui-hai, LI Jing-yuan, et al. Optimization of Purification Technology of Total Saponins from with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 4-7.
DOI:
BAI Dong, WANG Rui-hai, LI Jing-yuan, et al. Optimization of Purification Technology of Total Saponins from with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 4-7. DOI: 10.11653/syfj2013120004.
Optimization of Purification Technology of Total Saponins from with Macroporous Resins
Objective: To optimize purification technology of total saponins from Allium sativum with macroporous resin. Method: With transfer rate of total saponins as index
single factor tests were adopted to optimize purification process parameters
such as type of macroporous resin
adsorption capacity of resin (adsorption capacity
the concentration of sample solution
pH and adsorption speed) and elution performance (the concentration
flow rate and volume of eluent). Result: Optimized purification technology was as following:selected AB-8 macroporous resin
ratio of crude drug-resin 1.3:1
the concentration of sample solution 0.8 g·mL-1
pH 7
adsorption speed 2 BV·h-1
removed impurities with 30% ethanol
eluted with 5 BV of 95% ethanol
elution speed 3 BV·h-1.Under these conditions
purity of total saponins was over 65%. Conclusion: AB-8 macroporous resin could be adopted to purify total saponins from A. sativum
this optimized purification technology was stable and feasible.
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Related Author
LI Chun-hong
MEI Zhi-qiang
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FU Yan-ling
HU Kai-wen
Related Institution
Drug and Functional Food Research Center,Luzhou Medical College
Medical Molecular Biology Laboratory,Luzhou Medical College
College of Food and Biological Engineering,The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs, Chongqing Three Gorges University
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School of Pharmacy, Jiangxi University of Chinese Medicine