Determination of Alpha Tocopherol Content in  by HPLC

SU Kun; SHI Song-li; ZHENG Dong-hua; LI Ju-xin

doi:10.11653/syfj2013120070

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Determination of Alpha Tocopherol Content in by HPLC

更新时间：2024-01-04

- Determination of Alpha Tocopherol Content in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 70-72(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013120070
  CLC：
- Published：2013
- 稿件说明：
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SU Kun, SHI Song-li, ZHENG Dong-hua, et al. Determination of Alpha Tocopherol Content in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 70-72.
DOI：

SU Kun, SHI Song-li, ZHENG Dong-hua, et al. Determination of Alpha Tocopherol Content in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 70-72. DOI： 10.11653/syfj2013120070.

摘要

目的: 利用高效液相法

测定出蒙古扁桃中维生素E的含量。方法: 采用色谱为Wondasil C18(4.6 mm×250 mm

5 μm)

流动相为甲醇-水(98:2)

流速1.0 mL·min-1

柱温25℃

检测波长 300 nm

检测器温度为室温(25℃)。结果: α-维生素E在1~100 μg呈良好的线性关系

方程为Y=5 176.7X-7 645.8(r=0.999 6)

平均回收率为99.4%(n=3

RSD 1.740%)

蒙古扁桃桃仁中维生素E的含量在7.12~15.36 μg·g-1

以雅布格戈壁最高

固阳爬榆树最低。结论: 该法可用于测定蒙古扁桃桃仁中的维生素E的含量

结果准确可靠

快速灵敏。

Abstract

Objective: To determine and compare vitamin E content in five different regions of Amygdalus mongolica. Method: HPLC method was established for determining the content of vitamin E. Methanol-water (98:2) was used as the mobile phase at the flow rate of 1.00 mL·min-1. The chromatographic column was WondaSil-C18 (4.6 mm×250 mm

5 μm); the detection wavelength was set at 300 nm; the column temperature was kept at 25℃

and the ethanol was used as solvents. Result: The calibration curves of vitamin E was in good linearity over the range of 1-100 μg (r=0.999 6). The average recovery of vitamin E was 100.1% (n=3

RSD 1.740). Among five samples from different areas

the highest content of vitamin E is determined in the sample of Yabuge Gebi and the lowest is the one from Guyang Payushu. Conclusion: The method was proved to be rapid

accurate and credible.

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Related Author

ZHU Zhao-jing1

DENG Kai-ying2

WANG Xue-yong

CUI Guang-hong

HE Xi-rong

HUANG Lu-qi

WU Yong1

HE Fu-yuan1

Related Institution

Henan College of Traditional Chinese Medicine

ZHEJIANG A & F UNIVERSITY

Nanjing University of Traditional Chinese Medicine,Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

College of Pharmacy,Yanbian University,Yanji

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