Determination of the Total Saponins Content of

DOU Fang; XI Miao-miao; WEN Ai-dong

doi:10.11653/syfj2013120076

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Determination of the Total Saponins Content of

更新时间：2024-01-04

- Determination of the Total Saponins Content of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 76-78(2013)
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- DOI：10.11653/syfj2013120076
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- Published：2013
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DOU Fang, XI Miao-miao, WEN Ai-dong. Determination of the Total Saponins Content of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 76-78.
DOI：

DOU Fang, XI Miao-miao, WEN Ai-dong. Determination of the Total Saponins Content of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 76-78. DOI： 10.11653/syfj2013120076.

摘要

目的: 建立太白楤木根皮中总皂苷含量的测定方法。方法: 采用TLC薄层色谱法

以甲醇-氯仿-水 (6.5:3.5:1) 为展开剂展开后

用 10%硫酸乙醇溶液显色

并在105 ℃下加热进行鉴别。接着以香草醛-冰醋酸法显色

以屏边三七苷为对照品

在检测波长553 nm处对太白楤木总皂苷采用紫外-可见分光光度法进行含量测定。结果: 在TLC色谱中

供试品与对照品色谱相应的位置上

日光下显相同的紫色斑点;紫外灯(365 nm)下显相同的紫色荧光斑点。屏边三七苷在0.020 65~0.123 9 g·L-1线性关系良好(r=0.999 9)

平均加样回收率为100.20%

RSD 0.88%(n=6)

太白楤木根皮中总皂苷中总皂苷含量为61.30%。结论: 以太白楤木根皮总皂苷含量为主要评价指标

所建立的方法准确、简便、快速

重复性好

可作为太白楤木根皮中总皂苷含量控制方法。

Abstract

Objective: To establish the quality standard of total saponins of Aralia taibaiensis. Method: A. taibaiensis was identified by TLC and spots were visualized by heating silica gel plates sprayed with 10% H2SO4 in EtOH

and the content of total saponins of A. taibaiensis was determined by visible ultraviolet spectrophotometry. Result: The TLC spots of A. taibaiensis were clear and well separated. The linear ranges of total saponins was 0.020 65-0.123 9 g·L-1 (r=0.999 9)

and the average recovery rate was 100.20% (RSD 0.885%). Conclusion: The established standard is applicable for the quality control of A. taibaiensis.

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Related Author

DUAN Haohan

JIA Hao

LEI Jingwei

YANG Chunjing

XIE Caixia

GONG Haiyan

ZHU Beibei

WANG Yanjing

Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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