Objective: The research aimed to establish a high pressure digestion and a hydride-generation atomic fluorescence spectrometry (HG-AFS) method for the determination of selenium in Gastrodia elata and Quantianma capsules. Method: The samples

include G. elata herbs

Quantianma capsules and CRM Tea

Astragalus and Ginseng were prepared by high pressure digestion. The reaction medium was HNO3-HCl

which had reducing and interference diminishing effects. K3Fe (CN)6 was added to diminish the interference of coexist elements. Selenium in G. elata and Quantianma capsules is researched by HG-AFS. Meanwhile

selenium in the CRM Tea

Astragalus and Ginseng was detected in order to control the quality of the measured results. Result: There was a good linear relation between fluorescence intensity and concentration. The LOD was 0.16 μg·L-1. The precision and repeatability were satisfactory. The recovery was 98.26%-101.2% and RSD was 2.4%-4.4%. The method was used to detect the concentration of selenium in 22 batches of G. elata herbs and 5 batches of Quantianma capsules. The concentration of selenium in 22 batches of herbs is 0.012 4-0.660 mg·kg-1. The content was obviously different due to the different origins. As the origin of the Quantianma capsules is same

the concentration of selenium is 0.214-0.385 mg·kg-1. Conclusion: The method is easy and simple to handle. It has low cost and little pollution to environment. This method can be applied into the determination of selenium in G. elata and Quantianma capsules. It provides useful references for the quality control and further application of G. elata and Quantianma capsules.