Objective: To establish HPLC fingerprint of Herba Euphorbia Humifusae for quality control of the herbal medicine. Method: A Diamonsil C18 (4.6 mm×250 mm

5 μm) analytical column was used. The mobile phase was methanol-0.1% H3PO4 water. The elution was in gradient mode and detection wavelength was set at 280 nm. The column temperature was maintained at 32℃ and the flow rate was 0.8 mL·min-1. The analytical time was 60 min. Result: There were 24 common peaks in the fingerprint of 10 batches of Herba Euphorbia Humifusae samples

and each peak in the fingerprint was well separated under the chromatographic condition above. The similarity of the 10 batches of Herba Euphorbia Humifusae was not lower than 0.90. Conclusion: This method is available for the identification and quality control of Herba Euphorbia Humifusae.