LU Chun-ning, LIANG Yi-jian, CAO Yun, et al. Determination of Osthole in Huashe Jieyang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 98-100.
DOI:
LU Chun-ning, LIANG Yi-jian, CAO Yun, et al. Determination of Osthole in Huashe Jieyang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 98-100. DOI: 10.11653/syfj2013120098.
Determination of Osthole in Huashe Jieyang Capsule by HPLC
Objective: To establish a HPLC method for determining osthole in Huashe Jieyang capsule. Method: The Phenomenex Synergi C18 column (4.6 mm×250 mm
4 μm) was used with acetonitrile-water (62:38) as mobile phase. The UV detection wavelength was set at 322 nm and the flow rate was 1.0 mL·min-1. Column temperature was set at ambient temperature. Result: A good linearity was obtained as the content of osthole within the range of 0.077-0.772 μg (r=0.999 9) and the average recovery rate (n=6) was 105.0% with RSD 1.5%. Conclusion: The method is simple
available
reliable and accurate
so can be used to contro1 the content of osthole in Huashe Jieyang capsule.
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