Determination of the Related Substance in Alliin by RP-HPLC

HUANG Hong-yong; CUI Li-na; TANG Hui; HUANG Rui-ting

doi:10.11653/syfj2013120101

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Determination of the Related Substance in Alliin by RP-HPLC

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- Determination of the Related Substance in Alliin by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 101-104(2013)
- 作者机构：
- 作者简介：
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- DOI：10.11653/syfj2013120101
  CLC：
- Published：2013
- 稿件说明：
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HUANG Hong-yong, CUI Li-na, TANG Hui, et al. Determination of the Related Substance in Alliin by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 101-104.
DOI：

HUANG Hong-yong, CUI Li-na, TANG Hui, et al. Determination of the Related Substance in Alliin by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 101-104. DOI： 10.11653/syfj2013120101.

摘要

目的: 建立测定蒜氨酸中试原料药中有关物质的方法。方法: 采用RP-HPLC

DIKMA DiamonsilTM C18色谱柱(4.6 mm×250 mm

5 μm)

以水为流动相

流速0.8 mL·min-1

柱温25℃

检测波长220 nm。结果: 蒜氨酸与相邻杂质峰能完全分离

蒜氨酸在0.049 6~1.008 6 μg进样量与峰面积呈良好线性关系(r=0.999 6)

最低检测限为4 ng

精密度RSD 0.43%

平均回收率为99.22% (n=9)。结论: 该方法简便、准确、专属性好、灵敏度高

可用于控制蒜氨酸中试原料药的质量。

Abstract

Objective: To establish a method to determine the related substances of alliin. Method: RP-HPLC was adopted for the determination of the related substances of alliin. The chromatographic procedure was carried out with DIKMA DiamonsilTM C18 (4.6 mm×250 mm

5 μm) as an analytical column and water as mobile phase at a flow rate of 0.8 mL·min-1 at 25℃. The detection wavelength was set at 220 nm. Result: There was a good linear relationship within the ranger of 0.049 6-1.008 6 μg (r=0.999 6)

the limit of detection for alliin was 4 ng. The precision was 0.43%

the average recovery was 99.22% (n=9). Conclusion: The method is simple

accurate

specific and sensitiveness for quality control of alliin.

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Related Institution

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