UPLC Fingerprint Analysis of

XU Feng; YANG Ye; SHU Qiu-e; WANG Yuan-min; WANG Xiang-pei

doi:10.11653/syfj2013120108

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UPLC Fingerprint Analysis of

更新时间：2024-01-04

- UPLC Fingerprint Analysis of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 108-111(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013120108
  CLC：
- Published：2013
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XU Feng, YANG Ye, SHU Qiu-e, et al. UPLC Fingerprint Analysis of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 108-111.
DOI：

XU Feng, YANG Ye, SHU Qiu-e, et al. UPLC Fingerprint Analysis of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 108-111. DOI： 10.11653/syfj2013120108.

摘要

目的: 研究大血藤药材的UPLC指纹图谱。方法: 采用色谱法

Agilent ZORBAX RRHD Eclipse Plus C18色谱柱(2.1 mm×100 mm

1.8 μm)

洗脱系统0.05%乙酸水-甲醇梯度洗脱

检测波长280 nm

流速0.3 mL·min-1

柱温35℃。测定10批大血藤、2批鸡血藤及1批五香血藤的指纹图谱。结果: 建立了大血藤药材UPLC指纹图谱分析方法

确立了19个共有峰

指认了4个峰。10批大血藤药材相似度>0.837

大血藤与混淆品(鸡血藤和五香血藤)的指纹图谱具有明显差异。结论: 建立的方法快速、准确、重复性好

为评价和控制大血藤药材的质量提供参考。

Abstract

Objective: To establish a HPLC fingerprint method of Sargentodoxa cuneata. Method: Chromatography was carried out on a Agilent ZORBAX RRHD Eclipse Plus C18 column (2.1 mm×100 mm

1.8 μm) with gradient eluting system of 0.05% acetic acid water-methanol at a flow rate of 0.3 mL·min-1

detection wavelength was 280 nm

column temperature was at 35℃.Ten batches of S. cuneata

two batches of S. suberectus and a batch of Schisandra sphenunthera were determined for similarity comparison. Result: UPLC fingerprint analysis method of S. cuneata was established

there were 19 common peaks

four of them were identified.Similarity of 10 batches S. cuneata was more than 0.837

and fingerprint of S. cuneata had a significant difference to S. suberectus and S. sphenunthera. Conclusion: This method was rapid

accurate and reproducible

it could provide a reference for quality evaluation and control of S. cuneata.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Tianjin University of Traditional Chinese Medicine

Modern Research Center for Traditional Chinese Medicine，Shanxi University

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