Comparative Analysis of Total Ginkgolic Acids in  Leaves of Different Habitats

HUANG Ping

doi:10.11653/syfj2013120129

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Comparative Analysis of Total Ginkgolic Acids in Leaves of Different Habitats

更新时间：2024-01-04

- Comparative Analysis of Total Ginkgolic Acids in Leaves of Different Habitats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 129-131(2013)
- 作者机构：
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- DOI：10.11653/syfj2013120129
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- Published：2013
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HUANG Ping. Comparative Analysis of Total Ginkgolic Acids in Leaves of Different Habitats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 129-131.
DOI：

HUANG Ping. Comparative Analysis of Total Ginkgolic Acids in Leaves of Different Habitats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 129-131. DOI： 10.11653/syfj2013120129.

摘要

目的: 比较不同产地银杏叶中总银杏酸的含量。方法: 采用HPLC测定银杏叶中总银杏酸的含量。色谱条件为依利特Hypersil C18色谱柱 (4.6 mm×250 mm

5 μm)

流动相甲醇-1%冰醋酸水溶液(90:10)

流速1.0 mL·min-1

检测波长310 nm

柱温35℃

进样量20 μL

外标法计算含量。结果: 各产地银杏叶中总银杏酸含量差异较大

山东郯城总银杏酸含量最高(2.38%)

江苏邳州最低(1.28%)。结论: 不同产地银杏叶中总银杏酸含量差异明显

应选择使用。

Abstract

Objective: To study the change of total ginkgolic acids in Ginkgo biloba leaves of different habitats. Method: The content of total ginkgolic acids in G. biloba leaves was determined by HPLC. A Hypersil C18 column (4.6 mm×250 mm

5 μm) and the mobile phase of methanol and 1% acetic acid (90:10) were used; the flow rate was 1.0 mL·min-1

the wavelength was set at 310 nm. The content was calculated with external standard method. Result: The content of total ginkgoic acids in G. biloba leaves varied with different habitats. The content in the samples from Tancheng was the highest

while Pizhou is the lowest. Conclusion: The content of total ginkgolic acids in G. biloba leaves from different habitats is markedly different. We should have a choice in use.

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