CHEN Jun, LI Yao-hua, LI Xiao-long, et al. Determination of Content of Chrysoeriol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 138-140.
DOI:
CHEN Jun, LI Yao-hua, LI Xiao-long, et al. Determination of Content of Chrysoeriol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 138-140. DOI: 10.11653/syfj2013120138.
Determination of Content of Chrysoeriol in by HPLC
Objective: To establish the quantitative analysis method for determination of the content of chrysoeriol in Cardiospermum halicacabum by HPLC. Method: High perforamce liquid chromatogrphy was employed using Inertsil ODS-3C18Column(4.6 mm×250 mm
5 μm) and mobile phase comprising acetonitrile-0.1% phosphoric acid(35:65) at a floe rate of 1.0 mL·min-1 and detecting wavelength of 348 nm
the column temperature was room temoerature. Result: Chrysoeriol was linear in the range of 0.0134-0.268 μg(r=0.999 97) and the average recovery of chrysoeriol was 99.74% with RSD 2.1%. Conclusion: The method is simple
accurate
reproducible
which can be used for determination of the content of chrysoeriol in C.halicacabum.