YANG Guan-qi, DING Xiao-huan, YUE Zhi-jun, et al. Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 169-171.
DOI:
YANG Guan-qi, DING Xiao-huan, YUE Zhi-jun, et al. Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 169-171. DOI: 10.11653/syfj2013120169.
Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell
Objective: To investigate effect of Astragali Radix and Pseudostellariae Radix
Cortex Moutan Radicis and Salviae Miltiorrhizae Radix et Rhizoma
Arnebiae Radix and Leonuri Herba on m-RNA expression of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in mesangial cell of rats. Method: Using serum phamacological method
we got harmaeologieal serum of 3 couplet medicines.Cultured the glomerular mesangial cells and prolifelated them with angiotensin Ⅱ as stimulating factor.m-RNA expression of MMP-2 and TIMP-2 were measured by RT-PCR. Result: Effect of these 3 couplet medicines on MMP-2 were not statistically significant.Group of Astragali Radix and Pseudostellariae Radix obviously inhibited expression of TIMP-2
and there was significant difference by comparing with the modle group;Other groups also could inhibite the expression of TIMP-2
but there were not statistically significant. Conclusion: Couplet medicines of Astragali Radix and Pseudostellariae Radix could play prevention and treatment roles of glomeruselerosis by inhibiting gene expression of TIMP-2.