SUN Shu-yu, HE Xiao-juan, CHAI Wang, et al. Effects of Astragalus Polysaccharide on Treg Cells in Tumor-bearing Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 176-178.
DOI:
SUN Shu-yu, HE Xiao-juan, CHAI Wang, et al. Effects of Astragalus Polysaccharide on Treg Cells in Tumor-bearing Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 176-178. DOI: 10.11653/syfj2013120176.
Effects of Astragalus Polysaccharide on Treg Cells in Tumor-bearing Mice
Objective: To investigate the effects of astragalus polysaccharide(APS) on regulatory T cells (Treg) in tumor-bearing mice. Method: Mice were subcutaneously implanted with B16-F10 cells in the right back region. Then these mice were randomly divided into model group
cyclophosphamide(CTX) group
APS low dose group (0.15 g·kg-1)
APS high dose group (0.3 g·kg-1). Another ten normal mice were used as normal control. After 24 h
intragastric administration was performed. After 14 days of intragastric administration
tumors were removed and weighed
Treg of spleen were detected by flow cytometer(FCM)
mRNA expression of transforming grwoth factor-β(TGF-β) and interleukin-10(IL-10) in spleen was measured by real-time PCR. Result: The results showed that APS could inhibit the B16-F10 tumor growth
reduce the proportion of Treg in spleen(P<0.01)
decrease mRNA expression of TGF-β and IL-10 in spleen(P<0.05). Conclusion: The results indicated that APS might inhibit tumor growth partly by reducing the proportion of Treg in spleen and decreasing mRNA expression of TGF-β and IL-10 in spleen.