Antioxidant Activity in Leonuri Herba and Leonuri Fructus

WANG Can; LI Han-bing; QI Xiang-yun; MIAO Ming-san

doi:10.11653/syfj2013120179

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Antioxidant Activity in Leonuri Herba and Leonuri Fructus

更新时间：2024-01-04

- Antioxidant Activity in Leonuri Herba and Leonuri Fructus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 12, Pages: 179-181(2013)
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- DOI：10.11653/syfj2013120179
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- Published：2013
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WANG Can, LI Han-bing, QI Xiang-yun, et al. Antioxidant Activity in Leonuri Herba and Leonuri Fructus [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 179-181.
DOI：

WANG Can, LI Han-bing, QI Xiang-yun, et al. Antioxidant Activity in Leonuri Herba and Leonuri Fructus [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 179-181. DOI： 10.11653/syfj2013120179.

摘要

目的: 建立益母草、茺蔚子的体外抗氧化活性测定方法

对比益母草、茺蔚子抗氧化活性

为指导益母草和茺蔚子的临床合理用药提供支持。方法: 益母草、茺蔚子充分粉碎

室温下用75%乙醇超声30 min制备益母草、茺蔚子提取液

用二苯代苦味酰基自由基(DPPH·)法测定二者的抗氧化活性

并跟维生素E比较

评价益母草、茺蔚子的体外抗氧化能力。结果: 益母草、茺蔚子以及维生素E溶液对自由基清除率的半数有效浓度(EC50)分别为2.70

1.28

5.89 g·L-1

表明其抗氧化活性:茺蔚子提取液>益母草提取液>维生素E溶液;重复性考察结果表明实验结果重复性较好(益母草RSD 3.34%

茺蔚子RSD 2.25%)。结论: 益母草和茺蔚子在体外都具有较强抗氧化活性

且茺蔚子抗氧化活性强于益母草。

Abstract

Objective: To establish a method to assay antioxidant activity in vitro of Leonuri Herba and Leonuri Fructus

as a guide for clinical rational use of Leonuri Herba and Leonuri Fructus. Method: 75% ethanol extracting solutions of Leonuri Herba and Leonuri Fructus were prepared with ultraphonic for 30 min at 25℃

and their antioxidant capacity were evaluated with 1

1-Diphenyl-2-picrylhydrazyl radical-2

2-Diphenyl-1-(2

6-trinitrophenyl) hydrazyl (DPPH·) spectrometry

compared with vitamin E. Result: The extracts of Leonuri Herba and Leonuri Fructus both had good scavenging effects on DPPH·. IC50 of Leonuri Herba extract

Leonuri Fructus extract and vitamin E solution were 2.70

1.28

5.89 g·L-1

respectively. In other words the antioxidant capability was:Leonuri Fructus extract>Leonuri Herba extract>vitamin E. The repeatability inspection were:Leonuri Herba

RSD 3.34%; Leonuri Fructus

RSD 2.25%. Conclusion: Leonuri Fructus has a stonger antioxidant than Leonuri Herba in vitro.

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Related Institution

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, College of Pharmacy, Nanjing University of Chinese Medicine

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