TANG Han-qing, WEI Yi, LI Xiao-hua, et al. Effect of Fuzi Lizhong Decoction on AQP4 in Rats with Spleen Yang Deficiency Syndrome and its Antidiarrheal Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 222-225.
DOI:
TANG Han-qing, WEI Yi, LI Xiao-hua, et al. Effect of Fuzi Lizhong Decoction on AQP4 in Rats with Spleen Yang Deficiency Syndrome and its Antidiarrheal Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 222-225. DOI: 10.11653/syfj2013120222.
Effect of Fuzi Lizhong Decoction on AQP4 in Rats with Spleen Yang Deficiency Syndrome and its Antidiarrheal Mechanism
Objective: To observe effect of Fuzi Lizhong decoction on aquaporin 4(AQP4) in rats with spleen Yang deficiency syndrome and discuss its antidiarrheal mechanism. Method: One hundred Wistar rats were randomly divided into control group
model group
low-dose group
middle-dose group and high-dose group
20 rats each group. low-dose group
middle-dose group and high-dose group were ig given with Fuzi Lizhong decoction according to 10
20
40 g·kg-1 based on model group
once a day for four weeks.colon was collected as sample to examine content of AQP4 by ELISA method and expression of AQP4 mRNA by RT-PCR method. Result: Compared with control group
in model group both content of AQP4(13.25±4.22) ng·L-1 and expression of AQP4 mRNA (0.38±0.11)reduced (P<0.01 or P<0.05). Compared with model group
in middle-dose group both content of AQP4 (23.84±5.68)ng·L-1and expression of AQP4 mRNA (0.54±0.26)increased (P<0.05). Compared with model group
in high-dose group both content of AQP4 (28.98±6.12)ng·L-1and expression of AQP4 mRNA (0.62±0.32)increased significantly (P<0.01). Conclusion: Fuzi Lizhong decoction could recover content of AQP4 and expression of AQP4 mRNA
and high dose had a better effect. Fuzi Lizhong decoction could up-regulate expression of AQP4 mRNA and promote gastrointestinal reabsorption of water
which perhaps are one of its antidiarrheal mechanisms.
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