Objective:To investigate effect of different receiving solution on in vitro drug transdermal test. Method: 5-fluorouracil(5-FU) was applied as model drug

receiving solutions of a certain concentration of bovine serum albumin(BSA)

Brij98

poloxamer188

tween80

ethanol

γ-cyclodextrin and sodium dodecyl sulfate(SDS) fro transdermal test were prepared by physiological saline

the excised rat skins were treated with various additives for 12 h

followed by replacing those receiving solution with normal saline and adding saturated 5-FU into the donor cells to evaluate transdermal absorption of the model drug.Meanwhile

scanning electron microscope(SEM) was used to investigate morphology of the rat skins treated with those receiving solutions. Result: 3%BSA

6%Brij98 and poloxamer188

ethanol

tween80 and γ-CD with low concentration had no significant effect on in vitro drug transdermal absorption parameters

but they would exert an influence on the rat skins when the concentration of those four additives were high.In addition

ionic surfactant of SDS had an severely impact on the excised rat skins. Conclusion: Those additives

3%BSA

6%Brij98

the low concentration of tween80

poloxamer188

ethanol and γ-CD could be added to receiving solution in order to increase drug solubility in proper concentration.But ionic surfactant should be cautious to use as additives of receiving solution.