Objective:To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang. Method: HPLC was used with lichrospher C18column (4.6 mm×150 mm

5μm)

acetonitrile-0.1% phosphoric acid (46:64) with mobile phase. The flow rate was 1 mL·min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm. Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg (r=0.999 8) and the average recovery was 95.24% with RSD 1.33%. The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%

0.347%

0.373%. Conclusion: This method is simple

accurate with good reproducibility

and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.