QIU Hong-cong, LIU Bu-ming, SUN Cui. Optimization of Purification Process for Total Flavonoids from L.[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 34-36.
DOI:
QIU Hong-cong, LIU Bu-ming, SUN Cui. Optimization of Purification Process for Total Flavonoids from L.[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 34-36. DOI: 10.11653/syfj2013130034.
Optimization of Purification Process for Total Flavonoids from L.
Objective:To optimize purification process of total flavonoids from Baeckea frutescens L. by macroporous adsorption resin. Method: Different types of resin were selected with absorption and desorption ratio of total flavonoids as indexes
the content of total flavonoids was determined by UV
single factor test was adopted to investigate purification technology of total flavonoids by macroporous adsorption resin. Result: AB-8 type resin was adopted
its optimum process conditions were as followings:the concentration of sample solution 0.3 g·mL-1
absorption velocity 1 BV·h-1
sample volume 3 BV
eluted by 5 BV 50% ethanol at flow rate of 2 BV·h-1.Under these conditions
purity of total flavonoids was up to 52.33%. Conclusion: Optimized process was rational
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Related Author
LV Zi-ming
Chen Kai
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LIANG Jun-qing
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Ru YANG
Cong-yan LIU
Related Institution
Multi-component of Traditional Chinese Medicine (TCM) and Microecology Research Center, Jiangsu Province Academy of TCM
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine
Key Unit of Exploring Effective Substances of Classical and Famous Prescription,State Administration of Traditional Chinese Medicine of the People’s Republic of China
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