Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD

HU Zhi-fang; WANG Xiao-ping; CHEN Jian-zhang

doi:10.11653/syfj2013130072

您当前的位置：

首页 >

文章列表页 >

Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD

更新时间：2024-01-04

- Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 72-74(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013130072
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
HU Zhi-fang, WANG Xiao-ping, CHEN Jian-zhang. Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 72-74.
DOI：

HU Zhi-fang, WANG Xiao-ping, CHEN Jian-zhang. Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 72-74. DOI： 10.11653/syfj2013130072.

摘要

目的: 建立HPLC-ELSD测定地黄不同炮制品中单糖含量方法

研究不同炮制方法对熟地黄单糖含量的影响。方法: 采用高效液相色谱法

以蒸发光散射检测器进行检测

Prevail carbohydrate ES色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(75∶25)

流速0.8 mL·min-1

柱温30 ℃

ELSD条件漂移管温度65 ℃

空气流速2.5 L·min-1

增益1。结果: D-葡萄糖平均回收率为98.24%

RSD 2.18%;D-果糖平均回收率为98.37%

RSD 2.11%;生地、炆制地黄、清蒸地黄、酒炖地黄中单糖含量分别为1.62%

(27.22±2.74)%

(9.63±2.05)%

(16.97±2.62)%。结论: 该方法操作简便

可靠稳定

结果准确;地黄炮制品中单糖含量远远高于生地黄

炆制地黄中单糖含量高于其他两种炮制方法

为阐明其炮制机理提供实验依据。

Abstract

Objective:To establish an HPLC-ELSD method for determination of monosaccharide content in different processed Radix Rehmannia Praeparata and to study the effect of different processed method on monosaccharide content in Radix Rehmannia Praeparata. Method: HPLC-ELSD was applied to analyze the content of monosaccharide with prevail carbohydrate ES column (4.6 mm×250 mm

5 μm)

acetonitrile-water (75∶25) as mobile phase. The flow rate was 0.8 mL·min-1;column temperature was kept at 30 ℃;temperature of drift tube set at 65 ℃

gas flow 2.5 L·min-1and gain 1. Result: The D-glucose and D-fructose average recovery was 98.24% (RSD 2.18%) and 98.37% (RSD 2.11%) respectively. The content of monosaccharide in Radix Rehmanniae from Wenzhi

steaming and stewing by liqour were 1.62%

27.22%±2.74%

9.63%±2.05%

16.97%±2.62% respectively. Conclusion: The method is simple

accurate and reproducible. The content of monosaccharide in Wenzhi Dihuang was higher than that of steaming Dihuang and stewing by liqour. It provides an experimental basis to illustrate its processing mechanisms.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Astragnaloside IV in Baicaojing Oral Solution by HPLC-ELSD

Optimization of Water Extraction Technology for Qijiao Ointment by Orthogonal Test

Related Author

FAN Bin1

LIU Hong2

SUN Jian1

YAN Guang-li

WANG Jian-gong

FU Ke

YANG Yi

LI Lu

Related Institution

Henan College of Traditional Chinese Medicine

School of Pharmaceatical Sciences,Tianjin Medical University

Research Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰